Heavy metals in organs of gudgeon (Gobio gobio L.) from Vardar River, R. Macedonia

R. Nastova*, V. Kostov, I. Ushlinovska

Institute of Animal Science, “Ss. Cyril and Methodius” University, Blvd. Ilinden 92 A, 1000 Skopje, Republic of Macedonia (Manuscript received 4 August 2017; accepted for publication 13 November 2017)

Abstract. Ecotoxicological examinations based on seven heavy metals (Fe, Cu, Zn, Mn, Cd, Pb and Cr) concentrations in organs of gudgeon (Gobio gobio L.) caught by 14 monitoring points at Vardar River in Republic of Macedonia were performed. The organ’s samples elements were read-out on AAS in flame and graphite cuvette. Significant increase of Pb and Cd contents in liver, gills and gonads of fish sampled downstream Skopje and the town of Veles was found. It should be pointed out that at times Cd concentrations in muscles were as high as 0.518 mg/kg which is significantly over the maximally allowed concentrations for human nutrition.

Biodiversity of the macrozoobenthos in some protected marine areas along Bulgarian Black Sea coast

E. Petrova*, S. Stoykov

Institute of Fish Resources, 9000 Varna, Bulgaria
(Manuscript received 30 August 2017; accepted for publication 6 November 2017)

Abstract. The aim of this paper is to establish the biodiversity status of the macrozoobenthos in some protected marine areas (NATURA 2000) along the Bulgarian Black Sea coast. The observation stations in the period 2010-2012 were within the frames of the following habitats: 1160 – Large shallow inlets and bays; 1110 – Sandbanks, slightly covered by seawater and Sublittoral mussel banks on sediment. In general, the data shows steady state compared to the 1980s, except for the area near Kamchia River, characterized by low biodiversity due to water pollution from the river inflow (with contaminants from agricultural and industrial sources in the upper river stream) and less salinity leading to marine mussel species reduction. In the border area, near the Emona zone, the black mussel catches varied between 160-600 kg in 2005, while only single specimens were detected in 2013. In the same field, the yield of Rapana venosa was within the limits of 5-40 kg in 2005 and between 80-160 kg in 2013. The established variability showed a dynamic balance between Mytilus galloprovicialis and Rapana venosa that pose a challenge for future stock of the black mussel and point the need of regular monitoring.

Taxonomic composition of phytoplankton in Black Sea area in front of the Cape Galata (2008-2016)

D. Klisarova, D. Gerdzhikov

Institute of Fish Resources, 9000 Varna, Bulgaria
(Manuscript received 13 August 2017; accepted for publication 3 October 2017)

Abstract. In this article have been investigated the taxonomic structure of the phytoplankton communities developing in the Black Sea area in front of the Cape Galata. For the period 2008 – 2016, relatively high species diversity was found (161 species). Throughout the most of the year dominated class Dinophyceae (up to 54.17%). The number of phytoplankton species decreased with distance from the shore, but the percentage participation of the group of peridineas and diatoms in the general taxonomic composition grew. Only in class Prymnesiophyceae increased biodiversity was registered in the high seas

Inventory of the legal base for reclamation of lands disturbed by open-cast mining in Bulgaria

M. Banov*, V. Tzolova, I. Kirilov

N. Poushkarov Institute of Soil Science, Agrotechnologies and Plant Protection, 1080 Sofia, Bulgaria (Manuscript received 4 July 2017; accepted for publication 18 October 2017)

Abstract. Samples of geological materials, used for reclamation of land, disturbed by open coal mining have been investigated. The obtained results for the opportunities of biological regeneration of the lands fertility, reclaimed with these materials, put them in the following order: Pliocene clays from the region of Maritza Iztok (the biggest energy complex with the largest open coal mine in Bulgaria); marl materials from the area of the town of Pernik; and last – sulfur containing materials from the region of Chukurovo Mine, Sofia region. It is proposed that the period for biological reclamation, shown in Article 4, item 2 §(а) from Regulation No. 26/1996 for reclamation of disturbed areas, improvement of lands of low productivity, removal and utilization of the humus layer, must be changed from”5years”to”7-12years”for the lands reclaimed without humus and to”3-5years”for the lands reclaimed with of humus horizon used.

Saved СО2 emissions by using renewable sources for hot water yield in Bulgarian dairy farms

R. Georgiev1*, R. Slavov2, K. Peychev1, D. Georgiev1, S. Apostolov3, J. Ellingsen4, J. Tønnesen4

1Department of Agricultural Engineering, Faculty of Agriculture,Trakia University, 6000 Stara Zagora, Bulgaria
2Department of Animal Science – Ruminant Animals and Dairy Science, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria 3Bioselena Foundation for Organic Agriculture, Bulgaria
4Royal Norwegian Society for Development, Norges Vel, Norway

(Manuscript received 13 September 2017; accepted for publication 10 November 2017)

Abstract. In 2014 – 2015 installations for hot water yield from renewable energy sources were built and tested in three dairy farms in Bulgaria. These replace the traditionally used electricity on farms with the aim of decarbonising the energy production. The newly built installations contain three modules for heat yield – from recuperation of the heat from the milked milk, from the solar energy and from wood pellets. In the course of one year the energy obtained from the renewable sources has been measured and assessed. The present article assesses the ecological benefits of the separate renewable sources which are used to reduce СО2 emissions, the main greenhouse gas. For this purpose, the method of environmental life cycle analysis (LCA) and assessment of heat/hot water generating systems was used. Coefficients for calculating the primary energy of the saved or replaced energy, as well as their respective carbon ratios, specific for Bulgaria, were used. The results obtained are related to identifying the specific quantities of saved CO2 emissions from the renewable sources used on the experimental farms. It has been found that about 52-57% of CO2 savings are due to the pellets used, 34-42% to the solar heat collectors and about 9% to the recuperated heat from the produced milk.

Ecological analysis of the flora in the ‘Chinarite’ protected area – Rodopi municipality, Bulgaria

L. Dospatliev1*, M. Lacheva2

1Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria;
2Department of Botany, Faculty of Agronomy, Agricultural University, 4000 Plovdiv, Bulgaria

(Manuscript received 10 June 2016; accepted for publication 21 August 2017)

Abstract. The aim of the present study was to make an ecological analysis of the floristic composition in the protected area ‘Chinarite’ Rodopi municipality, Bulgaria in terms of its biological spectrum, biological types and phytogeographic elements and an assessment of the anthropogenic effect on it. In studied protected area were identified 147 species of vascular plants, of them 8 species included in the Red List of Threatened Species of the International Union for Conservation of Nature (IUCN). 73 medicinal species, 55 nectariferous plants and 10 tertiary relicts were recorded. The anthropogenic impact on the protected area is obvious and it has affected to the greatest degree the species composition of vascular plants, which encompasses a large number of weeds – 28 species,ruderals-29species,alien -5speciesandinvasive-6species.

Avifauna abundance and diversity in Jos wildlife park, Nigeria

B.T. Kwaga1, D. Iliya1, A. Ali1, D. Khobe2*

1Department of Forestry and Wildlife Management, Federal University of Technology, Yola, Nigeria 2Department of Animal Production, Adamawa State University, Mubi, Nigeria

(Manuscript received 1 June 2017; accepted for publication 30 August 2017)

Abstract. There is a great need to determine the abundance and diversity of any ecosystems biodiversity through its species composition and richness. This study compared the diversity and abundance of bird (avifauna) species in three habitats of the Wildlife Park, Plateau State, Nigeria. The three vegetation types (Gallery, Savannah and the Rocky areas) were surveyed. Fifteen (15) line transects (five in each of the habitats) were laid in the morning and evening hours. The data obtained were subjected to descriptive statistics (mean and percentages). Simpson’s diversity index was also employed in assessing the diversity of avifauna species in the study area. The result obtained indicated that a total of 616, 569 and 363 birds were recorded in the morning hours while a total of 117, 140 and 115 were identified in the evening. The results of birds species diversity (morning and evening) indicated that transects II (0.5956) and I (0.7407) for Gallery forests, transects II (0.6946) and III (1.000) for Savannah V (0.0784) and I (0.0752) for Rocky habitats, respectively, had the highest birds diversity. It is recommended that pastoralists and Plateau State Government should liaise and provide alternative grazing reserves to minimize the encroachment of the pastoralists into the Park and other protected areas.

Influence of basic agrotechnical activities on the productivity and yield of Triticum monococcum L.

S. Stamatov*, K. Uzundzhalieva, E. Valchinova, G. Desheva, P. Chavdarov, B. Kyosev, T. Cholakov, R. Ruseva, N. Velcheva

Institute of Plant genetic Resources „Konstantin Malkov”, 2 Druzhba, 4122 Sadovo, Bulgaria (Manuscript received 7 June 2017; accepted for publication 25 August 2017)

Abstract. The study was conducted during the period 2014 – 2016 in the experimental field of Institute of Plant Genetic Resources, Sadovo. The influence of the period of sowing, the predecessor and the fertilization on the elements of productivity and grain yield per hectare in einkorn wheat were studied. It was established that the structural elements of productivity – number of productive tillers, length of the spike, number of spikelets per spike, mass of central spike, number of grains in central spike, weight of the grains from the central spike are with the highest values in plants grown after predecessor peas, sown in autumn and fertilized with extra nitrogen. With least developed productive capabilities are the plant variants sown in spring after sunflower and without additional fertilization with nitrogen. With proved the biggest impact on grain yield per hectare is the date of sowing. The sources of variation: predecessor and combined effect of factors predecessor x fertilization show equal share of influence on the productivity of einkorn. The results from the study give science-based information on the most favorable time of sowing, fertilizing and predecessor in einkorn.

Animal hygiene assessment of microclimate in semi open free-stall barns for dairy cows

D. Dimov1*, Tch. Miteva1, I. Marinov2, Zh. Gergovska2, Т. Penev1, A. Enchev3

1Department of Applied Ecology and Animal Hygiene, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria 2Department of Animal Science – Ruminants and Dairy Farming, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria 3Enfo Agro LTD, Bulgaria

(Manuscript received 2 December 2016; accepted for publication 16 February 2017)

Abstract. The study was conducted in three semi open free-stall barns (B1, B2, and B3) for dairy cows with capacities for 120, 120 and 500 cows, respectively, from three different dairy farms (F-1, F-2 and F-3), situated in Central Southern Bulgaria. The investigated farms had the same production system – loose housing in semi open free-stall dairy barn. For each of the farms the main microclimatic parameters – air temperature, relative humidity and speed of airflow were recorded twice a month at 10.00 h 12.00 h, 14.00 h, 16.00 h and 18.00 h of the day inside the barns in three main technological zones – above the stalls, above manure and feed alleys and outside the buildings. It was found that: a) Microclimatic parameters (air temperature, air relative humidity and speed of airflow) in technological zones (above the stalls, the manure and feed alleys) of three semi open free-stall dairy barns meet the animal hygienic requirements for all seasons according to Regulation No. 44 (2006). Exceptions are some values of relative humidity in B1 and B2 in the spring, and in B1 in winter and summer, which are lower than the minimum humidity (50%) according to the standard. b) The investigated barns are characterized with poor insulation and do not provide enough isolation from the external ambient temperatures. With the exception of winter, the temperature of the air inside the buildings was lower than that outside, with minor differences for all seasons. The fans in the barns have no effect on the inside air temperature, especially in summer. There was a risk of higher temperatures mainly during the summer period. c) There is no significant difference between the average temperatures, air humidity and speed of airflow in all technological zones of the investigated barns. d) The largest and statistically significant is the difference between the relative air humidity outside and inside the building in Farm 3, followed by buildings in Farm 1 and 2, where the differences are smaller and statistically insignificant. e) Factor analysis revealed a significant effect: of the farm on temperature in the different technological zones in the barns (P <0.05-0.01); of the farm in the zones of manure and feed alleys (P <0.001) and less effect in the zone of stalls (P <0.05) on speed of airflow, the factor season on speed of airflow in all zones (P <0.001), the time of recording in the zone of manure alley on speed of airflow (P <0.05); the farm, season and time of recording on the humidity in the three technological zones (P <0.001).

Screening of cucurbitaceous rootstocks against root-knot nematodes (Meloidogyne spp.) and soilborne pathogens (Fusarium spp. and Pythium spp.)

V. Yankova*, D. Markova, N. Velkov, S. Masheva

Maritsa Vegetable Crops Research Institute, 32 Brezovsko shosse, 4003 Plovdiv, Bulgaria (Manuscript received 22 August 2016; accepted for publication 30 January 2017)

Abstract. One of the main problems in cucumber greenhouse production is control of soil-borne pathogens and root-knot nematodes. Grafting cucumber plants represent an alternative method to control that is safety and does not pollute the environment. Immune forms to these pests are not established, but in some studies are found sources belong to Cucurbitaceae family that possesses resistant or tolerant response. The aim of this study was to screening cucurbitaceous rootstock genotypes to root-knot nematodes (Meloidogyne spp.), Fusarium spp. and Pythium spp. During the period 2014-2015 in the Maritsa Vegetable Crops Research Institute, Plovdiv thirteen breeding materials belonging to Cucurbitaceae family were tested: Gergana, Kiara F1, TG, TD (Cucumis sativus); CM 720, SB-2, SB-3, Turban (C. maxima); Muskatna 51-17, Carotina, (C. moschata); Turban × Muskatna 51-17, CM 720 × Carotina (C. maxima × C. moschata F1); Local (Lagenaria siceraria). Two parallel trials were performed in greenhouse conditions. Local isolates of pests were used for the screening tests. Plants were grown in pots and inoculated with mixed infection of Fusarium spp. and Pythium spp. In trial with root-knot nematodes the plants were inoculated with 6000 second stage juveniles (J2). The response was recorded 60 days after inoculation. Results indicated that Carotina was resistant when the pots were inoculated with Meloidogyne spp. Resistant response to soil-borne pathogens possesses cucumber lines TG and TD and Lagenaria. Tested cucurbitaceous material can be used directly for grafting cucumber plants, and also be able to used as a basis for starting breeding program for rootstocks with tolerance to Meloidogyne spp., Fusarium spp. and Pythium spp.