Phenotypic diversity in six-rowed winter barley (Hordeum sativum L.) varieties

  1. Dyulgerov, B. Dyulgerova

 

Institute of Agriculture, 8400 Karnobat, Bulgaria

(Manuscript received 24 July 2017; accepted for publication 19 January 2018)

 

Abstract. The objective of this investigation was to study the phenotypic diversity among six-rowed winter barley varieties. The study was conducted in the Institute of Agriculture – Karnobat, during the period 2014/2015-2015/2016. The diversity among 22 barley varieties was estimated using principal component analysis and cluster analysis. Maximum variation was found for grain weight per a spike and grain number per a spike. The presence of significant variation among the evaluated six-rowed winter barley varieties suggests an opportunity for improvement of grain yield through using promising genotypes as parents in the winter barley improvement program.

Agronomic performance of mutant lines of winter two-rowed barley

  1. Dyulgerova, D. Valcheva, N. Dyulgerov

 

Institute of Agriculture, 8400 Karnobat, Bulgaria

(Manuscript received 24 July 2017; accepted for publication 19 January 2018)

 

Abstract. The aim of this investigation was to study agronomic and morphological traits of winter two-rowed barley mutant lines in the M6 and M7 generations. Eight mutant lines, their parent – breeding line 244D and national standard cultivars – Obzor and Emon were evaluated in Complete Block Design with four replications. The research was conducted in the 2013/2014, 2014/2015 and 2015/2016 growing seasons in the experimental field of the Institute of Agriculture – Karnobat, Southeastern Bulgaria. The characters studied included days to heading, plant height, lodging, peduncle length, spike length, awn length, spikelet number per spike, grain number per spike, grain weight per spike, 1000 grains weight, grain yield, protein content, extract content and grading (>2.5mm). Mutant lines M1/3, M1/5 and M1/217 produced a significantly greater grain yield than the parent and standard cultivars. The improvement of grain yield was associated with increasing of spike length and grain weight per spike. The studied mutant lines were characterized with a high yield ability combined with other valuable agronomic traits and can be used in a breeding program for developing winter malting  barley varieties.

Yield and coefficient of ecological valence of spring barley in the regions of Sadovo and Karnobat, Bulgaria

  1. Neykov, T. Mokreva

 

1Department of Plant Genetic Resources, Institute of Plant Genetic Resources, 4122 Sadovo, Bulgaria

2Faculty of Economics, Agricultural University, 4000 Plovdiv, Bulgaria

(Manuscript received 27 April 2017; accepted for publication 21 September 2017)

 

Abstract. The study was conducted to examine the yield and the coefficient of ecological valence of spring barley in the regions of Sadovo and Karnobat. Seven spring barley cultivars were planted in the first and second ten days of March, in comparative cultivars trials on the block method in four repetitions of an area of 10 m2, with sowing rate of 450 germinating seeds per m2. They were assessed on the standard for the French cultivar Josefin used of the Executive Agency of Varietal Testing, Field Inspection and Seed Control. The statistical processing of the results was carried out by ANOVA. The coefficient of ecological valence (W) is calculated.  Cultivars grown in the region of Sadovo showed better ecological plasticity, but gave lower yields, in the region of Karnobat were more productive but difficult to adapt to environmental conditions. Suitable cultivars for growing spring barley in the regions of Sadovo and Karnobat are Barke, Scarlett and Bodega. They exceed the standard variety Josefin in yield with proven differences at both test sites.

Нordein polymorphism between spring barley cultivars by SDS-PAGE electrophoresis

N. Neykov1*, S. Doneva2

1Department of Plant Genetic Resources, Institute of Plant Genetic Resources, 4122 Sadovo, Bulgaria 2Dobrudzha Agricultural Institute, 9521 General Toshevo, Bulgaria

(Manuscript received 27 April 2017; accepted for publication 28 September 2017)

Abstract. In this study reserve endosperm proteins, hordeins of seven spring barley cultivars with different origin: Zernogradskii (Russia), Bodega, Fink, Scarlett and Barke (Germany), Josefin and Astoria (France) were fractionated and characterized by SDS-PAGE electrophoresis. On the basis of the obtained spectra 19 bands (D + C + B) with different relative electrophoretic mobility and intensity were identified. The electrophoresis profiles of the groups D-, C- and B- hordein are designated as separate types (models) using the index corresponding to hordein blocks. We have established one profile type for D-hordein (D1), two- for C-hordein (C1, C2), and five – for B-hordein (B1, B2, B3, B4, B5). Based on these results hordein formulas (configurations) of accessions are constructed, which enable the expression of specific varietal characteristics and prove the existence of the inter allelic variation (hordein polymorphism) due to the presence or absence of protein components and their different electrophoretic mobility in the profiles of D-, C- and B-hordein.

Study on the loss of accuracy of AC-method for milk yield control in sheep

D. Dimov*, P. Zhelyazkova, A. Vuchkov

Department of Animal Sciences, Faculty of Agronomy, Agricultural University, 4000 Plovdiv, Bulgaria (Manuscript received 30 June 2017; accepted for publication 5 September 2017)

Abstract. The aim of this study was to investigate the loss of accuracy of AC-method for milk yield control in sheep under the conditions of threefold milking per day. Test day milk yield records of 113 ewes raised on sheep farm of the Agricultural University in Plovdiv collected according to official A4 method were used. Two breeds were raised at the university experimental farm: White Maritza (49 ewes) and Patch Faced Maritza (64 ewes). Predicted milk yield data on test day were simulated using different prediction coefficients (calculated at morning, noon or evening milk recording). Database includes 2577 predicted milk yield records on test day. The loss of accuracy using AC method is accumulated in two ways: by milk yield prediction coefficients on test day and milk yield calculations during milking period. Loss of accuracy (LA1) in prediction of test day milk yield by prediction coefficients varies from 10.02% to 12.74% according to type of milk recording (morning, noon or evening). Three factors such as level of test day milk yield, type of milk recording and animal have larger influence on LA1, 27.70%, 21.99% and 19.32%, respectively, of total variation. Loss of accuracy (LA2) in the calculated total milked milk per milking period on the basis of predicted test day milk yield according to the morning, noon and evening milk recordings compared with A4 method are 7.04%, 8.03% and 6.05%, respectively. Depending on the type of milk recording on the test day (morning, noon and evening), 46.88% to 56.15 % of the observations of LA2 fall within the scope of ±5%. Rank correlations in the ranking of ewes in all years and different designs of AC method have high values from 0.891 to 1.000.

Variation in the agronomic and morphological traits in spring barley

N. Dyulgerov, B. Dyulgerova*

Institute of Agriculture, 8400 Karnobat, Bulgaria
(Manuscript received 4 July 2017; accepted for publication 27 October 2017)

Abstract. The study was conducted to examine the variation in the agronomic and morphological traits in spring barley. For this purpose, 22 lines from the ICARDA High Input Barley Program for favorable environment and 3 check varieties (Rihane-03, VMorales and Veslets) were tested in an alpha-lattice design with two replications at the Institute of Agriculture – Karnobat, Bulgaria in 2014 and 2015 growing season. The traits days to heading, plant height, number of tillers per plant, flag leaf length, flag leaf width, spike length, awn length, peduncle length, spikelet number per spike, grain number per spike, grain weight per spike, 1000 grains weight, grain yield, powdery mildew (Erysiphe graminis f. sp. hordei), net blotch (Pyrenophora teres f. teres) and stripe rust (Puccinia striiformis f. sp. hordei) infection were studied. Significant differences between lines for all studied traits were found. The number of fertile tillers per plant was significantly positively correlated with grain yield. Lines expressed higher grain yields, shorter stem, better tolerance to net blotch and stripe rust than Bulgarian check variety Veslets were identified. These genotypes can, therefore, be used as parents for the improvement of spring barley.

In vitro propagation of oil-bearing rose (Rosa damascena Mill.)

V. Badzhelova*

Institute of Roses, Essential and Medical Cultures, Kazanlak, Bulgaria (Manuscript received 12 June 2017; accepted for publication 25 August 2017)

Abstract. The purpose of this research is to develop effective protocol for clonal micro propagation of the oil-bearing rose (Rosa damascena Mill.). Explants used in the experiment are 1.0 to 1.5 cm long nodal segments from specially cultivated mother plants. These explants were subjected to a combined method of sterilization with 0.2% solution of HgCI2 for 3 minutes followed by 0.50% solution of NaClO for 20 minutes and 0.25% solution of NaClO for 30 minutes. The best results of multiplication were obtained in basic MS medium with added BAP quantity of 0.5 to 3.0 mg/L. After being separated from the mother plants the young shoots are placed directly in compost mixture for rooting and adaptation. Thus the process of multiplication is shortened, avoiding the period of in vitro rooting.

Parthenogenetic responsiveness of sunflower hybrid combinations with expressed tolerance to herbicides

M. Drumeva*, P. Yankov

Department of Plant Production, Faculty of Shipbuilding, Technical University Varna, 1 Studentska, 9010 Varna, Bulgaria (Manuscript received 6 March 2017; accepted for publication 3 July 2017)

Abstract. For accelerated development of fertility restorer lines with imidazolinone tolerance in sunflower (Helianthus annuus L.), the gamma-induced parthenogenesis, which combines classical breeding approaches and in vitro techniques, was applied. As initial materials six imidazolinone-tolerant hybrid combinations (F1203, F1216, F1246, F1248, F1295 and F1332), obtained from Dobrudzha Agricultural Institute, General Toshevo, were used. To initiate parthenogenetic development mixed pollen from different fertility restorer lines was applied. The mixed pollen was irradiated with a dose of 600 Gy. A total of 166 parthenogenetic immature embryos were obtained, and 84 young plants developed from them, 42 of the plants were fertile. Best parthenogenetic response, expressed as a number of embryos obtained, was observed by hybrid combination F1295, followed by hybrid combination F1332. The lowest parthenogenetic responsiveness is reported in hybrid combinations F1246 and F1248, where the total number of parthenogenetic embryos obtained was 5. In hybrid combinations F1295 and F1332, as well as in F1216, the method of gamma-induced parthenogenesis can be efficiently used for accelerated production of parental lines for heterosis breeding in sunflower. In hybrid combinations F1203, F1246 and F1248, which showed low parthenogenetic responsiveness, conventional methods for developing lines from these hybrids are recommended.

Evaluation of high yielding mutants of Hordeum vulgare cultivar Izgrev

B. Dyulgerova*, N. Dyulgerov

Institute of Agriculture, 8400 Karnobat, Bulgaria

(Manuscript received 7 February 2017; accepted for publication publication 31 May 2017)

Abstract. Seeds of Hordeum vulgare L. cultivar Izgrev were treated with different concentrations of sodium azide to induce genetic variability for the selection of genotypes with improved traits. After passing through different stages of selection, 18 promising mutants were selected for further studies. Eighteen mutants and their parent and national standard cultivar Veslets were evaluated in Complete Block Design with four replications. The research was conducted in 2013 – 2014 and 2014 – 2015 growing seasons in the experimental field of the Institute of Agriculture Karnobat, Southeastern Bulgaria. The characters studied included days to heading, plant height, lodging, peduncle length, spike length, awn length, spikelet number per spike, grain number per spike, grain weight per spike, 1000 grains weight and grain yield. Wide variation among mutant lines was observed for different traits. Mutant lines M4/16 and M3/14 produced significantly greater grain yield than the parent and standard cultivar. Positive changes in lodging tolerance, grain number per spike, grain weight per spike, 1000 grains weight were also observed. This study showed positive effects in the use of mutation in inducing improvement for grain yield and some yield related traits.

Characterization of a new winter malting barley cultivar Ahil

B. Dyulgerova*, Dr. Vulchev, T. Popova

Institute of Agriculture, 8400 Karnobat, Bulgaria

(Manuscript received 7 February 2017; accepted for publication 30 May 2017)

Abstract. Ahil is a new winter malting barley cultivar developed by the method of experimental mutagenesis at the Institute of Agriculture, Karnobat, Bulgaria. Biological and agronomic characteristics of the cultivar were also investigated for several years and at the Executive Agency of Varietal Testing, Field Inspection and Seed Control trails in the period 2012 – 2013. Averaged for 6 locations in Bulgaria grain yield of the new cultivar was significantly higher than the yield of standard cultivars with about 5%. At the Institute of Agriculture grain yield was 17% higher than the average standard for the period of 5 years. The cultivar has high winter hardness, lodging resistance, resistance to powdery mildew and medium resistance to brown rust. Ahil has good malting quality.