Haematological investigations upon acute intoxication with carbofuran in dogs

R. Binev*, I. Valchev, R. Russenov, Y. Nikolov

Department of Internal Non-Infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria (Manuscript received 7 September 2016; accepted for publication 19 December 2016)

Abstract. Experiments for monitoring of changes in haematological parameters were carried out in dogs after acute intoxication with the carbamate insecticide carbofuran (Carbosan 35 ST). The studies involved one control and 6 experimental groups of dogs (total n=42), treated once orally with increasing doses of the preparation via oesophageal probe: 0.525 mg/kg (experimental group I), 1.05 mg/kg (experimental group II), 2.1 mg/kg (experimental group III), 3.5 mg/kg (experimental group IV), 5.25 mg/kg (experimental group V) and 10.5 mg/kg (LD50), (experimental group VI), corresponding to 1/20, 1/10, 1/5, 1/3, 1/2 and LD50, oral doses for albino rats. Blood samples were obtained from v. antebrachi cephalica or v. jugularis in the course of 3 consecutive days prior to the treatment (hours -48, -24 and 0) and on post treatment hours 1, 3, 5, 7, 24 and 48 from all groups for analysis of blood cell counts (RBC), white blood cell counts (WBC), haemoglobin content (HGB), haematocrit (HCT), differential white cell counts (DWC) erythrocyte sedimentation rate (ESR). It was established that the tested carbamate insecticide caused decrease RBC, HGB and LYM, and increased the levels of WBC and banded neutrophils between post treatment hours 1 and 24; afterwards, the studied parameters regained the respective control values.

Clinical toxicological investigations on acute carbofuran intoxication in quails (Coturnix coturnix)

R. Binev1*, I. Valchev1, R. Mihaylov2, Y. Nikolov1

1Department of Internal Non-Infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria 2Department of Animal Morphology, Physiology and Nutrition, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

(Manuscript received 7 September 2016; accepted for publication 19 October 2016)

Abstract. The present studiy was conducted to evaluate the toxic effects of the carbamate insecticide carbofuran (Carbosan 35 ST) after experimental acute intoxication in quails (Coturnix coturnix). Experiments for monitoring of changes in clinical indices and some haematological parameters. Quails were divided into 5 groups: one control and 4 experimental. They were treated by increasing single doses of the tested pesticide: 1.05 mg/kg (experimental group I), 2.1 mg/kg (experimental group II), 5.25 mg/kg (experimental group III) and 10.5 mg/kg (experimental group IV), corresponding to 1/10 LD50, 1/5 LD50, 1/2 LD50 and LD50 oral doses for albino rats, respectively. In three consecutive days prior to the treatment (hours –48, –24 and 0) and 1, 3, 5, 7, 24 and 48 hours thereafter, the clinical status was registered to evaluate rectal body temperature, faeces excretion, locomotion, perception etn. and were obtained blood samples from v. subcutanea ulnaris or v. metatarsea ulnaris for analysis of haemoglobin content (HGB). It was found out that the tested carbamate insecticide had some toxic effects manifested clinically with hyperaemia, arexia, difficulty in focusing the eyes, salivation with thick saliva discharge, diarrhoea, generalised tremor, clonic tonic spasms (especially of cervical muscles), depression and hypochromaemia. The described changes were the most obvious between post treatment hours 1 and 3, and then the studied parameters regained their control values. The tolerated dose of carbofuran was 1.05 mg/kg (1/10 LD50 for albino rats), the minimum toxic dose was 2.1 mg/kg (1/5 LD50 for albino rats), and minimum lethal dose – 10.5 mg/kg, equal to LD50 for albino rats.

Reproductive performance of breeding rabbits fed by graded levels of cassava (Manihot esculenta) leaf meal

T. Ahemen1*, I.I. Bitto1, O.I.A. Oluremi2

1Department of Animal Breeding and Physiology, University of Agriculture, Makurdi, Nigeria 2Department of Animal Nutrition, University of Agriculture, Makurdi, Nigeria

(Manuscript received 12 March 2016; accepted for publication 21 September 2016)

Abstract. Twenty breeding does and 4 rabbit bucks aged 28 weeks, weighing between 1622 to 1746g ,were used to investigate the reproductive performance of breeding does fed by graded levels of cassava leaf meal (CLM). The rabbits were randomly assigned to four dietary treatments viz: T1 (control), T2, T3 and T4 with CLM inclusion levels of 0%, 10%, 20%, and 30%, respectively, after balancing for live weight. Each rabbit was individually caged and served as a replicate. The feeding trial lasted for 17 weeks. The results showed that does on diet T2 (100%) achieved the highest conception rate, followed by T3 (75%) and T4 (75%) and the least in T1 (66.67%). Average daily weight change and change in weight during pregnancy were the highest (p<0.05) in T4. Litter size at birth was significantly (p<0.05) lower in T4 (2.67), while litter size at weaning was significantly (p<0.05) higher in T3 (3.67). Average birth and weaning weights were significantly (p<0.05) higher in treatment T4. Percentage of still birth was higher in T2 (60%) while pre-weaning mortality was the highest in T1 (5). It was concluded that the inclusion of up to 30% of CLM in rabbit diet may support reproductive performance in female rabbits. It is recommended that further studies on the feeding potential of cassava leaf meal at higher levels of inclusion on reproductive performance be investigated.

Lysozyme levels in haemolymph of worker bees (Apis mellifera L.) from bee colonies with different degree of expression of hygienic behaviour

S. Lazarov1, I. Zhelyazkova1*, D. Salkova2, R. Shumkova3, S. Takova4

1Department of Animal Science-Non-ruminants and other Animals, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria 2Bulgarian Academy of Sciences, Institute of Experimental Pathology and Parasitology, Fauna and circulation of parasites, 1000 Sofia, Bulgaria 3Experimental Station in Animal Husbandry and Farming, 4700 Smolyan, Bulgaria
4National Diagnostic Scientific Research Veterinary Medical Institute, 1000 Sofia, Bulgaria

(Manuscript received 1 June 2016; accepted for publication 10 August 2016)

Abstract. A total of 24 bee colonies of apiaries with different business orientation were tested for the degree of expression of hygienic behaviour by modified method, different from the traditionally used for this purpose method. To outline the test field a square sized 5 x 5 cm was used, stuck onto a section of a honey comb with sealed worker brood (the area bounded by the stencil is equal to 100 worker bee cells). The brood in the outlined square is killed by a thin entomological needle by jabbing the sealed cells, without destroying their caps. Depending on the time and extent of cleaning bee colonies are divided into 3 groups: super hygienic – colonies which of the 24th hour after the jabbing have uncovered and cleaned over 95% of the outlined area; hygienic – colonies which on the 48th hour after the jabbing have uncovered and cleaned over 95% of the outlined area; non- hygienic – colonies which have cleaned less than 95% of the cells in the area on the 48th hour. From each bee colony samples of worker bees (200-250 pcs.) have been taken and haemolymph obtained. The amount of lysozyme has been defined at the Reference Laboratory “Honeybee health” at the National Diagnostic Scientific Research Veterinary Medical Institute – Sofia by the method of Motavkina et al. (1979), modified by Kostov et al. (1983). The results obtained show different values for the amount of lysozyme in haemolymph of worker bees, depending on the degree of expression of their hygienic behaviour – 10.49 ± 1.86 μg/ml for the group of super hygienic colonies; 9.11 ± 1.37 μg/ml for the group of hygienic ones; 15.22 ± 2.37 μg/ml for the group of non-hygienic bee colonies, respectively. The established values range from 4.59 μg/ml to 38.28 μg/ml, the greatest variation being in the group of non-hygienic colonies. The data suggests that in positive direction compared to the average for the model is the deviation of LS-means of bee colonies with low level of hygiene (non-hygienic). The reported LS-estimates suggest that in the non- hygienic bee colonies there is a tendency of increase the lysozyme content in the haemolymph.

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Plastid pigments quantity and some physiological parameters related to photosyntetic processes in triticale grown for green biomass

H. Nedeva*, R. Ivanova, H. Yancheva

Crop Science Department, Faculty of Agronomy, Agricultural University, 12 Mendeleev, 4000 Plovdiv, Bulgaria
(Manuscript received 20 May 2016; accepted for publication 6 June 2016)

Abstract. The aim of the study was to establish the amount of plastid pigments and some physiological parameters related to photosynthetic processes in two triticale cultivars, grown for biomass under different nitrogen fertilization rates and harvesting stages in the region of Southern Bulgaria. The research was carried out in the field of Crop Science Department at the Agricultural University, Plovdiv, during the period 2013 – 2015. The experiment was set after the block method with four replications, and 20m2 for each experimental plot. Two triticale cultivars – Musala and Attila grown for green biomass at different fertilizer rates (N , N , N , N , N ) have been tested. The two cultivars are harvested in heading formation and milk maturity stages. The physiological parameters – 0 120 160 200 240 intensity of transpiration, photosynthesis rate and the amount of plastid pigments (Chlorophyll A, Chlorophyll B, Carotene) have been observed at different cultivars and harvesting stages. In both tested cultivars in heading stage the transpiration intensity was higher when rates of N were used, while in milk 200 maturity stage the highest values were in variant with N . The photosynthesis rate at both cultivars in heading stage was increased with increase of the 240 nitrogen rate. In the stage of milk maturity the values of some indices decreased in both cultivars. The lowest content of plastid pigments in leaves of Musala cultivar was received in the control variant. It was observed that the increase of the nitrogen rate leads to increasing the pigments content. The highest content of pigments in Attila cultivar was received when N was applied.
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Optimization of formulations with balanced biochemical composition and possibilities for their extrusion

M. Ruskova1*, T. Petrova1, I. Bakalov1, N. Penov2, A. Simitchiev2

1Food Research and Development Institute, 154 V. Aprilov, 4000 Plovdiv, Bulgaria
2Department of Canning and Refrigeration Technology, Technological Faculty, University of Food Technologies, 26 Maritza, 4000 Plovdiv, Bulgaria
(Manuscript received 10 June 2015; accepted for publication 9 May 2016)

Abstract. Combinations of different raw materials (beans, einkorn wheat, and buckwheat) for obtaining formulations with high protein content and balanced amino acid composition were studied using simplex centroid design. The target functions for optimization were content of protein, sulfur-containing amino acid methionine and cysteine, lysine, and tryptophan. The optimal area of combinations of raw materials in the food formulas with balanced biochemical composition was obtained. Optimized ternary mixture consisting of 50% bean, 40% einkorn wheat, and 10% buckwheat with different moisture content (16, 22, and 28%) has been extruded in a laboratory single screw extruder (Brabender 20 DN, Germany). Extrusion parameters were as follows: feed screw speed 50 rpm, die diameter 3 mm, screw compression ratio 2:1, temperature profile 100/140/160ºC, screw speed 160 rpm. The three extrudates with different initial moisture were evaluated by sectional expansion index, water absorption index, water solubility index, and density, with the aim of choosing the best treatment. The results demonstrated that the 22% initial moisture content yielded an extrudate with good physicochemical characteristics overall but an optimization study is needed to confirm this.
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Influence of mineral nitrogen and organic fertilization on the productivity of grain sorghum

S. Enchev*, G. Kikindonov

Agricultural Institute, 9700 Shumen, Bulgaria

Abstract. The production of grain sorghum has increased in recent years. The modern varieties are hybrids with high productivity potential appropriate for application of intensive forage grain production technologies. The increased requirements for ecological production necessitate optimization of the nutrition parameters in the conditions of unstable agriculture with unbalanced soil nutrient substance accumulation. The effect of nutrition with mineral nitrogen fertilizer and organic fertilizer Humustim on the productivity of 6 varieties of Euralis Semences, popular in the practice, was tested during 2011 – 2013. In the various climatic conditions fertilization is a highly effective factor for productivity increase. The use of organic fertilizers such as Humustim widens the possibilities of the ecological production.

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Energy productivity, fertilization rate and profitability of wheat production after various predecessors I.Energyproductivityofwheat

Z. Uhr1*, E. Vasileva2

1Institute of Plant Genetic Resources, 2 Druzhba, 4122 Sadovo, Bulgaria
2University of Agribusiness and Rural Development, 78 Dunav, 4000 Plovdiv, Bulgaria

Abstract. As a consequence of a long-year comparative study of quality, productivity and physiological characteristics of Bulgarian varieties of common winter wheat(Triticumaestivum),wecarriedouta studyontheadaptationofmoderngenotypestotherequirementsofsustainableagricultureandrespectivelytheir effectiveness of breeding methods with respect to these requirements. In the course of this study were also accumulated additional data concerning the influence of the predecessor and nitrogen fertilizer rate on energy productivity of crop, energy and recyclable efficiency of fertilization and profitability of production. In two consecutive publications we summarize and share with the scientific community the data that we have received. The results are up-to-date and useful in both theoretical and practical aspects. The analysis is based on data from field fertilizer experiments carried out with predecessor cereal – regular crop of sorghum, millet, maize and legumes after predecessor–separate sowing of chickpeas. Energy productivity is calculated as a function of yield (grain, straw and total biological yield, (t/ha), multiplied by the energy equivalent unit (MJ/kg). Maximum values of gross energy productivity by grain yields are reported by fertilization rate with 0.18 t/ha nitrogen fertilizer after cereal predecessor and after 0.06 t/ha after legumes. After cereal predecessor the effect of increasing the fertilizer rate to 0.18 t/ha N improves energy productivity of grain by 160% compared to the control N0. After legumes predecessor at N 0.0 t/ha energy productivity with grain was 12%, and in N 0.06 – 21% higher than the maximum (at 0.18 t/ha) after cereal predecessor. Legume predecessor unifies differences between genotypes and fertilizer levels on energy productivity of grain and increases energy productivity of straw by 16% on average. Maximum values of gross energy productivity of straw are reported in fertilization with 0.12 t/ha after wheat and 0.24 t/ha fertilizer nitrogen after legume predecessor. Increasing the fertilizer rate from 0.0 t/ha to 0.18 t/ha nitrogen reduces the difference in the gross energy productivity of crops after legumes and cereal predecessor from 105% in 0.0 t/ha to 10% in 0.18 t/ha. The differences between both varieties regarding the studied parameters are statistically significant at P < 0.001.

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Phosphorus fractions in alluvial meadow soil after long-term organic-mineral fertilization

S. Todorova*, K. Trendafilov, M. Almaliev

Department of Agro-chemistry and Soil Science, Faculty of Agronomy, Agricultural University, 12 Mendeleev, 4000 Plovdiv, Bulgaria

Abstract. The aim of our study was to investigate phosphorus fractions, their content and distribution in alluvial meadow soil after continuous fertilization. Our research was focused on a long-term field experiment with different variants of fertilization set in 1959 in the Experimental field of Agricultural University of Plovdiv. Since 2006 the fertilization has been discontinued. In 2011, we collected soil samples from two depths (0 – 30 and 30 – 40 cm) and from the following variants: variant 1 (control, without fertilization), variant 2 (N50P30K20), variant 3 (N25P15K0 + 6t/da manure), variant 4 (N50P0K20). The studied soil is characterized by relatively good natural phosphate regime. The amount of available phosphates determined by the Egner-Rheem method is high – average 16.76% of the total phosphorus. Over 50% of the mineral phosphates in the soil are in available (in varying degree) form. Relatively high is the amount of organic phosphates, which during the mineralization can also be involved in the plant nutrition. The amount of available phosphates (according to Egner-Rheem) is determined to the highest degree by the total phosphorus in soil, which in turn is highly correlated with the content of hardly soluble calcium phosphates.

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Ethological and haematological indices in yearling sheep fed various dietary nitrogen sources

I. Varlyakov*, V. Radev, Т. Slavov, R. Mihaylov

Departmet of Morphology, Physiology and Animal Nutrition, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

Abstract. A physiology experiment was conducted to evaluate the effects of diet supplementation with non-protein nitrogen compounds Optigen and urea. The trialcomprisedthreeperiods:control–yearlingsheepwerefed rationwithbarley,sunflowermealandmeadowhay;firstexperimental–thesamerationwas supplemented with 12 g Optigen and second experimental – the ration was supplemented with 10.6 g urea. The addition of Optigen resulted in increased appetite and faster consumption of the food as seen from lower feeding time and increased rumination time. The welfare of animals was good as seen from the low ratio of time spent ruminating in standing position to total rumination time and the dominating values of rumination and rest times without statistically significantdifferencesbetweenexperimentalperiods.Therewerenodifferencesbetweentimesspentstandingand loafing,includingmovementinsidethe box, which is an indirect index for lack of discomfort and reliable testimony for the conditions of the present experiments. The blood picture results allowed affirming that the health status of experimental animals was within the reference limits. Proofs in support of this thesis were the fact that lower red blood cell counts were compensated by the higher haemoglobin content, whereas white blood cell counts were normal and accompanied by nutritional leukocytosis during all three study periods.

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