Effects of antioxidant (Salvia officinalis L. extract) on in vitro fertilization and micromanipulation intracytoplasmic sperm injection in Albino mice

H. Al-Ahmed, R. Mahood, G. Bonev, S. Georgieva

Abstract. The aim of this experimental study was to assess the effects of antioxidant (Salvia officinalis L. extract) on fertilized percentage of sperm. Ability and efficacy of treated sperms by two methods of fertilization (IVF and ICSI) in albino mice male. Ten males treated intra peritonially with S. officinalis L. for 35 days, sperm motility, sperm dead and abnormality were (86.43%±6.54, 17.50%±3.01, 13.39±2.04) respectively compared to control group (72.31%±3.92, 26.23%±2.94, 24.33%±2.91). Thirty adult mice females were treated with ECG and hCG to obtain super ovulation to increase numbers of oocytes. Females were sacrificed by dislocation of cervical vertebrate. Oviducts were removed and placed in a sterile disposable Petri dish containing 1ml Phosphate buffer solution (PBS). After collection of sperms from treated males, and follow method of in vitro fertilization and intracytoplasmic sperm injection, results of oocytes fertilized by IVF were 65.97%, while oocytes fertilizing by ICSI method were 61.53% compared to control sperms without treated by Salivia Officinalis (43% for IVF and 45.45% for ICSI).

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