Influence of various cryoprotectants on the sperm mobility of Muscovy semen before and after cryopreservation

A study for influence of five cryoprotectans – glycerol, dimethyl sulfoxide (DMSO), ethylene glycol, 1,3 propandiol and polyethylene glycol in different concentrations from 3%, 5% and 7% with use of HIA-1 and AU extenders on the mobility of Muscovy spermatozoa after freeze-thaw was carried out. The semen was collected with artificial vagina from 9 one-year-old Muscovy drakes by using a female as a teaser twice a week. The sperm was diluted with HIA-1 and AU extenders with 15% egg yolk (v/v) at the ratio of 1:3 (semen:extender), respectively, and divided equally. Cryoprotectant was added in one of following concentrations 3-5-7% into each semen sample, respectively, a process of equilibration in a refrigerator followed at 4 0 C for 60 min. Afterwards they were directly dropped in concave cavities of dry ice at -790 C for 10 min. The semen pellets were placed in an atmosphere of liquid nitrogen (LN2) vapors for 5 – 10 min and finally they were put in cryotubes and plunged into liquid nitrogen. The pellets were kept frozen in the LN2 container for at least 2 months before being thawed for evaluation. The sperm samples were thawed at 420 C with HIA-1 and AU extenders, respectively. Comparatively the highest sperm mobility was established at using 5% and 7% glycerol and 7% DMSO. Both HIA-1 and AU extenders are suitable to semen dilution. Cryopreservation of Muscovy semen caused damages in the morphological integrity of sperm cells.

Influence of various cryoprotectants on the sperm mobility of Muscovy semen before and after cryopreservation

V. Gerzilov