Investigations on haematological parameters and bone marrow morphology in broiler chickens with experimental aflatoxicosis

I. Valchev1*, D. Kanakov1, Ts. Hristov1, L. Lazarov1, N. Grozeva2, Y. Nikolov1

1Department of Internal Non-Infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria 2Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria

Abstract. In this experiment, the toxic effects of AFB1 on some haematological parameters were investigated in chickens, namely haemoglobin content (HGB), red blood cell counts (RBC), white blood cell counts (WBC), platelet counts (PLT), haematocrit (HCT), differential white blood cell counts (WBC%), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). The experiments were conducted with five groups of ten 7-day-old Cobb broiler chickens in each. The formed groups were: group I – control, fed a standard compound feed; group II – experimental, whose feed was supplemented with 1 g/kg Mycotox NG; group III – experimental, receiving 0.5 mg/kg AFB1; group IV – experimental, receiving 0.8 mg/kg AFB1 and group V – experimental, supplemented with 0.5 mg/kg AFB1 and 1 g/kg Mycotox NG. The experiment’s duration was 42 days. Haematological analysis performed on the 21st day of the trial showed reduction in RBC, PLT, HGB and HCT and increased total WBC. The percentages of the different leukocyte classes (differential leukocyte counts) demonstrated increased proportion of pseudoeosinophils and lower percentages of lymphocytes and monocytes. The observed changed tended to become more pronounced on the 42nd day of the experiment. There were no statistically significant changes in MCV, MCH, and MCHC, as well as in eosinophil and monocyte percentages between control and treated groups (р>0.05). The supplementation of the feed with mycosorbent – Mycotox NG (experimental group V) reduced partly the harmful effect of AFB1 on studied haematological indices. There were no substantial changes in studied parameters in chickens from experimental groups I and II.