Effect of experimentally induced aflatoxicosis on haematological parameters and bone marrow morphology in mulard ducks

  1. Valchev1, N. Groseva2, D. Kanakov1, Ts. Hristov1, L. Lazarov1, R. Binev1

1Department of Internal Non-Infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria,

2Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria

 

DOI: 10.15547/ast.2018.03.040

(Manuscript received 12 June 2018; accepted for publication 20 August 2018)

 

Abstract. In this experiment, the toxic effects of AFB1 on some haematological parameters were investigated in mulard ducks, namely red blood cell counts (RBC), haemoglobin content (HGB), haematocrit (HCT), platelet counts (PLT), white blood cell counts (WBC), differential white blood cell counts (WBC %), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). Experiments were conducted with 4 groups of 20 10-day-old mulard ducks each. The groups were as followed: group I – control, fed a standard compound feed according to the species and the age; group II – experimental, whose feed was supplemented with 0.5 mg/kg AFB1, group III – experimental, supplemented with 0.8 mg/kg AFB1 and group IV – experimental, supplemented with 0.5 mg/kg AFB1 and 2 g/kg Mycotox NG. The duration of the experiment was 42 days. Haematological analysis performed on the 21st day of the trial showed reduction in RBC, HCT, HGB and PLT and increased total WBC in groups II and III. The percentages of the different leukocyte classes (differential leukocyte counts) demonstrated increased proportion of heterophils and lower percentages of lymphocytes. The observed changed tended to become more pronounced on the 42nd day of the experiment. There were no statistically significant changes in MCV, MCH, and MCHC, as well as in eosinophil, basophil, and monocyte percentages between control and treated groups (р>0.05). The supplementation of the feed with mycosorbent – Mycotox NG (experimental group IV) reduced partly the harmful effect of AFB1 on the studied haematological indices.

Haematological investigations upon acute intoxication with carbofuran in dogs

R. Binev*, I. Valchev, R. Russenov, Y. Nikolov

Department of Internal Non-Infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria (Manuscript received 7 September 2016; accepted for publication 19 December 2016)

Abstract. Experiments for monitoring of changes in haematological parameters were carried out in dogs after acute intoxication with the carbamate insecticide carbofuran (Carbosan 35 ST). The studies involved one control and 6 experimental groups of dogs (total n=42), treated once orally with increasing doses of the preparation via oesophageal probe: 0.525 mg/kg (experimental group I), 1.05 mg/kg (experimental group II), 2.1 mg/kg (experimental group III), 3.5 mg/kg (experimental group IV), 5.25 mg/kg (experimental group V) and 10.5 mg/kg (LD50), (experimental group VI), corresponding to 1/20, 1/10, 1/5, 1/3, 1/2 and LD50, oral doses for albino rats. Blood samples were obtained from v. antebrachi cephalica or v. jugularis in the course of 3 consecutive days prior to the treatment (hours -48, -24 and 0) and on post treatment hours 1, 3, 5, 7, 24 and 48 from all groups for analysis of blood cell counts (RBC), white blood cell counts (WBC), haemoglobin content (HGB), haematocrit (HCT), differential white cell counts (DWC) erythrocyte sedimentation rate (ESR). It was established that the tested carbamate insecticide caused decrease RBC, HGB and LYM, and increased the levels of WBC and banded neutrophils between post treatment hours 1 and 24; afterwards, the studied parameters regained the respective control values.