Application of mobile fluorescence spectroscopy in determination of varietal differences in Spinach (Spinacia oleracea L.)

V. Slavova*

Maritsa Vegetable Crops Research Institute, Department of Plant Breeding Plovdiv, Bulgaria, Agricultural Academy Bulgaria

(Manuscript received 5 September 2023; accepted for publication 27 February 2024)

Abstract. The present study aims to establish the application of fluorescence spectroscopy as a mobile field method in the determination of varietal differences in Spinach (Spinacia oleracea L.). The experimental studies were conducted on site at the farm where the spinach accessions were grown. Fluorescence analysis was conducted using a light source emitting at a wavelength of 285 nm and a mobile fiber-optic experimental setup developed by the authors. The subjects of this research are leaves from Rembord, Marishka, Marquisa, and Victoria. The correlation between the emission signals of the samples was established. This discovery enables the successful application of mobile fluorescence spectroscopy as a rapid tool for determining the origin of unknown leaves. The results from the experiment can be used to optimize the timing for analyzing varietal differences in spinach genotypes after harvest. Fluorescence spectroscopy in a fiber-optical configuration will support the process of determining the belonging of a specific variety to a given variety, even for samples of unknown origin, when it is necessary to qualify the result of Š°ccessions in a short time.