Karyotype variability of the Ukrainian Mountain-Carpathian sheep breed

K. Typylo*

Institute of Animal Breeding and Genetics nd.a. M.V. Zubets of National Academy of Agricultural Sciences, Chubinsk Boryspil district, Kyiv region, 08321, Ukraine

(Manuscript received 5 September 2019; accepted for publication 25 November 2019)

Abstract. The main purpose of this work was to describe the karyotype variability of the Ukrainian Mountain-Carpathian sheep breed. Cytogenetic studies were carried out on a group of 25 sheep from that breed. The cultivation of lymphocytes, preparing the cytogenetic samples, classification and registration of chromosome aberrations were held using conventional methods. It was established that the number and structure of the chromosomes of sheep of the Ukrainian Mountain-Carpathian breed correspond to the norm typical for this species of animals. Chromosomal variability of the studied population includes 19.22% of numerical and structural aberrations. By the number and structure of the chromosome set the Ukrainian Mountain-Carpathian sheep breed does not differ from other breeds of sheep. Accurate identification of individual chromosomes with routine coloring allows using cytogenetic studies in breeding practice in sheep breeding.

Heterosis manifestations for spike productivity traits in durum wheat

R. Dragov*
Field crops institute (FCI), 6200 Chirpan, Bulgaria
(Manuscript received 5 September 2019; accepted for publication 18 October 2019)

Abstract. The aim of the study is to investigate the heterosis manifestations in durum wheat for quantitative traits related to spike productivity. Diallel cross includes five modern varieties of durum wheat: Victoria (BG), Deni (BG), Superdur, Progress (BG), Predel (BG). The heterosis manifestations of the ten crosses are traced for the traits: spikelet number per spike, number of kernel per spike, grain weight per spike and thousand kernel weight. The experiment is conducted in the 2014-2016 period in the experimental field of FCI- Chirpan. Standard technology for the cultivation of durum wheat is applied. The trials are organized in a randomized block design with three replications. Of each replication 30 plants are randomly picked and harvested for biometric analysis. The mean values by years from the F1 spike biometric measurements are included in the statistical analysis to determine the mid parent and better parent heterosis. For spikelet number per spike, seven crosses show high parent heterosis in the first year, four in the second and six in the third year. In all years, hybrids with a variety of Victoria have more spikelet number per spike and show better parent heterosis. For the trait kernel number per spike it is observed that combinations with Deni variety in most cases have better parent heterosis. In all years, the cross Superdur x Predel indicates high parent heterosis for this trait. For grain weight per spike most of the combinations exhibit high levels of better parent heterosis. When the Deni variety is used as female parent, all hybrid combinations show high levels of heterosis for the trait grain weight per spike. In regard to the thousand kernel weight different heterosis levels are observed, with only negative values in the first year. In the other two years in hybrid combinations there is positive heterosis in one year and negative in another year. The participation of the Deni variety in hybrid combinations leads to better parent heterosis for this trait. The data allow the use of these crosses directly in the breeding of durum wheat to increase individual traits and/or increase productivity.

Assessment of bread wheat (Triticum aestivum L.) genotypes based on their agronomic characters and tolerance to biotic stress

İ. Öztürk1*, A. Şen2, T.H. Kılıç1, Ş. Şili1

1Trakia Agricultural Research Institute, Edirne, Turkey
2Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey

(Manuscript received 15 April 2019; accepted for publication 10 September 2019)

Abstract. The aim of the study was to investigate the occurrence and severity of biotic stress factors, quality and agronomic parameters and its effect on yield and quality character of wheat genotypes. The experiment was conducted at Trakia Agricultural Research Institute, Edirne experimental area, during 2013-2015 cropping years. Grain yield, days of heading, plant height, 1000-kernel weight, test weight, protein ratio, leaf rust, stripe rust, Septoria leaf spot and relationship among these parameters were investigated. Based on a two years data, apart from protein content, other parameters showed significant difference (p<0.05). When compared to the mean yields in both cropping years, the genotypes Gelibolu and BBVD-3, BBVD-4, BBVD-9 genotypes were determined the better-adapted genotypes under target environment. Cultivar Aldane and TCI011322-8 line was detected as very tolerant to leaf rust and stripe rust in both years under the infection of field condition. Aldane had higher protein ratio, 1000-kernel weight and test weight. Grain yield was positively correlated with test weight and negatively correlated with foliar diseases. A highly significant negative correlation was determined between grain yield and plant height in both growing years. Leaf rust and Septoria tritici leaf disease had negative effect on protein ratio in both years. There was a significant positive relation between 1000-kernel weight and test weight. Results of the study indicated that based on biotic stress factors BBVD-6, BBVD-7 and TCI011322-8 are promising lines that need to be improved a bit further.

Preservation and study of old local forms of the so called “vineyard peach” (Prunus persica L.) in the Shumen region, Bulgaria

  1. Vasilev1, S. Malchev2, A. Zhivondov2


1“Konstantin Preslavsky” University of Shumen, 115 Universitetska Str., 9700 Shumen, Bulgaria

2Fruit Growing Institute, 12 Ostromila Str., 4004 Plovdiv, Bulgaria


(Manuscript received 20 June 2019; accepted for publication 15 August 2019)


Abstract. During the period 2015-2017, scientific expeditions were conducted in the region of the town Shumen, located in the north-eastern part of Bulgaria, mainly around the village Madara where the concentration of natural forms derived from seedlings is the greatest. The purpose is to discover and research old local forms of the so called “vineyard peach” and to preserve them for possible future inclusion in breeding programmes to create new cultivars using more extended genepool. As a result, three forms of “vineyard peach” were selected from the local populations. The fruits of the three local forms are small and with primitive taste qualities but fit for human consumption. Two of the forms have white fruit flesh and one is with green. Two of the peach forms have vigorous growth, but one of the forms with white fruit flesh is of weak vigour. The three forms are spontaneous natural products obtained through long-term seed propagation. The trees are old but vital and without visual damage by diseases and pests, regularly bear fruits and have good annual growth despite competition from weeds, bushes and trees.

Relationships between milk yield, quality and coagulation properties with kappa-casein (к-CN) genotypes of Bulgarian Rhodope cattle breed

  1. Mehandzhiyski1, Т. Angelova2, D. Yordanova2, J. Krastanov2


1Research Centre of Livestock Breeding and Agriculture, 4700 Smolyan, Bulgaria

2Agricultural Institute, 6000 Stara Zagora, Bulgaria


(Manuscript received 12 June 2019; accepted for publication 10 August 2019)


Abstract. The aim of the present study was to establish the association between different к-CN genotypes with milk yield, quality and coagulation properties in dairy cows of Bulgarian Rhodope breed. For determination of milk proteins polymorphism and its relationship with individual coagulation properties of milk and its quality, 136 milk samples from Bulgarian Rhodope dairy cows reared in 4 farms were collected. The visit to the farms and the taking of the milk samples took place between May and June 2009. Animal milk is based on daily milking on the relevant control day of the current lactation. During the morning milking, 50mL samples were collected without preservative, stored in cooling bags and analyzed in the specialized lab at the Agricultural Institute, Stara Zagora by Computerized Renneting Metter. The evaluated parameters of milk coagulation properties were: rennet coagulation time (RCT, min); curd firming time (K20, min) and curd firmness (A30, mm). Milk proteins polymorphism was identified by tissue samples collection from 136 cows. To this end, specialized pliers and marks with a vial containing desiccant were used. With this technique, the tissue sample is obtained and sealed at the time of identification of the animal. After collection, tissue samples were shipped to the University in Padova, Italy for PCR-RFLP analysis. DNA was purified using a Maxwell®16 Tissue DNA purification kit (Promega) according to the manufacturer’s instructions. Data were analyzed using descriptive statistical analysis (SYSTAT 13). The results obtained give reason to the following conclusions: The highest milk yield was established in cows from the AH genotype – 16kg, whereas the lowest value was detected in cows from the BH genotype – 12.83kg. Homozygous cows from the BB genotype produced milk with the highest fat content – 5.11%, whereas heterozygous from the BH genotype: milk with the lowest protein content – 3.16%. The cows from the AH genotypes were outlined with the lowest milk fat and protein content. The longest rennet coagulation time (RCT) was observed in АА and АН genotypes, the longest curd firming time (К20) – in genotype AH, the highest curd firmness (А30) – in the milk of cows from the AB and BB genotypes.

Population status, breeding policy and perspectives for the development of the Danubian horse breed

  1. Vlaeva1, N. Lukanova 2, M. Popova3

1Sub-department “Horse breeding”, Department of Animal husbandry – Non-ruminants and other animals,

Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

2Institute of Animal Science, 2232 Kostinbrod, Bulgaria

3 Department of Animal Sciences, Agricultural University, 12 Mendeleev Str., 4000 Plovdiv, Bulgaria

(Manuscript received 12 June 2019; accepted for publication 22 July 2019)


Abstract. The population status and breeding policy of the Danubian horse breed were studied for a relatively long period, from 1953-2017. The study traced the change in population number of the breed in decades and by different categories of animals. The analyses show a strong reduction in the number of Danubian horses in all categories. The small number of newborn foals is associated with the lowering number of breeding mares especially after the 1980s. In the last decade, according to an officially published bulletin by the breeding organization on the other hand, there was an increase in the number of mares and stallions and inconsistent with that number of breeding horses, newly born foals. In a historical aspect, the breeding policy of the Danubian horse showed some interesting and unpublished so far facts. Those facts are related, on the one hand, with the origin of the mares that became founders of families and, on the other hand, with the use of stallions of different breeds for input of purebred animals.

Monitoring of genealogical structure development of East Bulgarian breed horses originating from stallions of other sporting breeds

  1. Sabeva1, M. Popova2

1Department of Animal Sciences, Agricultural Institute, 9700 Shumen, Bulgaria

2Department of Animal Sciences, Agricultural University, 4000 Plovdiv, Bulgaria

(Manuscript received 6 June 2019; accepted for publication 26 July 2019)


Abstract. The origin of horses from the East Bulgarian breed, which descent from the other sport breeds stallions used for grading has been investigated. Genealogical structure development was traced by pedigree analyses of 1123 horses, born during the period from 2000 to 2018. The East Bulgarian Horse Association set of statistical data, concerning stallions’ breeding activity and productive life of their progeny were used. Two new lines with founders Hanoverians Da Kapo (GER) and Eistanzer (GER) were set up during the last four generations. Nowadays the line of stallion Da Kapo (GER) has developed in second and third generation after the founder and the line of stallion Eistanzer (GER) – in fourth generation after the founder. A small bulk of genealogical groups with origin traced to stallions Ladykiller, Cottage Son, Cor de la Bryere and Alme Z have developed.

Emergence rate of seedlings from hard okra seed coats and seedling growth of some genotypes of West African okra (Abelmoschus caillei)

P P.M. Jonah1, G.K. Farauta3, Y. M. Kwaga1, H.E. Shehu1, N.M. Fakuta4, B.P. Mshelmbula2

1Department of Crop Science, Adamawa State University, Mubi, Nigeria
2Department of Botany, Federal University, Lafia, Nassarawa State, Nigeria
3Department of Agricultural Technology, Faculty of Education, Adamawa State University, Mubi, Nigeria
4Rubber Research Institute of Nigeria, Gum Arabic Sub-station, Gashua, Nigeria

(Manuscript received 12 March 2019; accepted for publication 30 April 2019)

Abstract. In 2017 and 2018 cropping season, field experiment was conducted at the Food and Agricultural Organization and Tree Crop Plantation, Department of Crop Science, Adamawa State University, Mubi using fifteen genotypes of West African okra. The study was undertaken to study the emergence rate of seedlings from hard okra seed coats, seedling growth and their development. The seeds of the genotypes were soaked in NaCl solution for 24 hours to accelerate the breaking of seed dormancy and to ease seed germination. Tough seed coat usually impairs seed germination by establishing a permeability barrier which can interrupt water uptake required for imbibition, radicle and seedling emergence. The combined analysis results revealed a highly significant (P≤0.01) difference among these genotypes with respect to days to first and 50% flowering including days to first harvest. Furthermore, accession 3 (NG/SA/DEC/07/0448) and accession 11 (Yar kwadon) flowered earlier, had shortest days to first harvest, recorded the highest number of seedlings and emergence percentage than the other genotypes studied.


Races of bacterial spot pathogen infecting genus Capsicum in Bulgaria

K. Vasileva1, N. Bogatzevska2

1Maritsa Vegetable Crops Research Institute, 32 Brezovsko Shosse Str., 4003 Plovdiv, Bulgaria
2Institute of Soil Science, Agrotechnologies and Plant Protection “N. Pushkarov”, 7 Shosse Bankya Str., 1331 Sofia, Bulgaria.

(Manuscript received 27 February 2018; accepted for publication 21 April 2019)

Abstract. The causative agents of bacterial spot disease infecting pepper are the species X. euvesicatoria and X. vesicatoria common in the typical pepper growing areas (Northern: Black Sea Costal and Central; Southwest; Southern – Upper Thracian valley) in Bulgaria. The pathogens refer to pepper P pathotype (22 strains) and pepper-tomato РТ pathotype (52 strains). The natural population of X. еuvesicatoria is heterogeneous of pathotype and physiological races. Widespread is race P6 in the P of the pathogen, followed by race P1. Single strains are assigned to P3, P9 and P10. In PT, races P0, P1, P4, P5 are differentiated, the dominant race in PT is the P4 race in combination with the tomato race T2. For the first time in Bulgaria in the natural population of X. vesicatoria, are detected strains which infect only pepper, differentiated are races Р0, Р2 and Р3. The population of X. vesicatoria PT prevails in private gardens and vegetable areas near tomatoes. Differentiated races are P1, P3 and P4 in combination with tomato races T1, T2 and T3. Race P3 occurs in P and PT pathotype.

Variation and stability of the advanced bread wheat (Triticum aestivum L.) genotypes under various environment conditions in Trakia region

İ. Öztürk1*, T. Kahraman1, R. Avcı1, A. Şen2, T.H. Kılıç1

1Trakia Agricultural Research Institute, Edirne, Turkey
2Department of Biology, Faculty of Science, İstanbul University, İstanbul, Turkey

(Manuscript received 10 October 2018; accepted for publication 24 January 2019)

Abstract. Various environmental conditions can cause some biotic and abiotic stress factors and reduce yield and quality of wheat. This research was carried out to determine the effect of the environmental conditions on yield, some agronomic, and leaf rust disease of the bread wheat genotypes in four different locations in Trakia region, Turkey. The experiment was established with 25 genotypes in randomised complete block design (RCBD) with 4 replications in 2008-2009 cycles. Grain yield, days to heading and maturity, plant height, lodging resistance, leaf rust and relationship among characters were investigated. The temperature and rainfall were scored at GS24-33, GS34-43, GS45-75 and GS77-89 growth stages and compared with the investigated traits. According to the results, significant differences among locations and some investigated parameters were found based on rainfall and temperature with yield and other investigated traits. The mean yield of the genotypes was 5851 kg ha-1, and CMSW97WM518S-12 had the highest yield with 6601 kg ha-1. Tekirdağ was the highest yielding location with 7022 kg ha-1, followed by Edirne (5808 kg ha-1) and Kırklareli (5754 kg ha-1). Due to various environment conditions infection of the leaf rust varied and there were differences among locations. Cultivar Aldane and three lines were resistant to leaf rust. Positive relation between yield with plant height and lodging resistance was found and this means that genotypes which have tall plant had higher yield potential. Rainfall at GS24-33 (r= 0.855) and GS34-43 (r= 0.911*) had a significant and positive effect on grain yield. Increase of temperature during the shooting, heading and grain filling period decreased grain yield, so negative association between grain yield and temperature at GS34-43 (r= -0.761), GS45-75 (r= -0.635) and GS77-89 (r= -0.815) was found. The overall evaluation suggested that increase of temperature from shooting up to grain filling decreased the grain yield duration of the maturating stage. Rainfall during the vegetative growth phase increased grain yield and biomass of plant of genotypes.