I. Valchev1*, N. Grozeva2, D. Kanakov1, Y. Nikolov1

1Department of Internal Non-infectious Diseases, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria 2Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria

Abstract. Morphological changes in the pancreas of broiler chickens were followed out after experimental aflatoxicosis В1. Also, the possibility to prevent the deleterious effects of AFB1, through dietary supplementation of Mycotоx NG (Ceva Sante Animale, France) was evaluated. The 42-day experiments were carried out with 50 Cobb broiler chickens, divided in 5 groups of 10 chickens: Group I – control; Group II – supplemented with 1 g/kg Mycotox NG; Group III – supplemented with 0.5 mg/kg aflatoxin B1; Group IV –supplemented with 0.8 mg/kg aflatoxin B1; Group V –supplemented with 0.5 mg/kg aflatoxin B1 and 1 g/kg Mycotox NG. Gross pancreatic changes in broilers treated at 0.5 mg/kg AFB1 comprised generalised vascular hyperaemia, interlobular oedema, initial disintegration of glandular acini. Birds treated at 0.8 mg/kg AFB1 exhibited complete glandular acini disorganisation, and mononuclear cell infiltrations. The supplementation with Mycotox NG alleviated the frequencty and severity of observed histological lesions: slight interlobular oedema, generalised hyperaemia and early granular dystrophy of the cytoplasm of Langerhans islet cells.