Concentration of sulfur-containing amino acids at turkey broilers during and after muscle dystrophy, fed with deficient feed supplemented with oxidised fat

K. Stoyanchev

Department of Internal Diseases and Clinical Toxicology, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria

(Manuscript received 5 April 2016; accepted for publication 7 June 2017)

Abstract. The aim of the present study was to reproduce experimentally muscular dystrophy in 50 broiler turkeys, through early nutrition with a diet deficient in vitamin E, selenium and sulfur-containing amino acids, supplemented with oxidised fat and to study blood plasma sulfur containing amino acids (methionine and cysteine). The experiments were conducted with 1 day-old broiler turkeys. By the 3rd day of life, they were divided into 40 experimental (II group) and 10 control birds (I group); the latter were fed a standard compound feed, whereas the former group received a diet deficient in sulfur-containing amino acids methionine and cysteine (reduced up to 50%), vitamin E, and Se (from 0.2 mg/kg in standard feed to 0.01 mg/kg), further supplemented with oxidized fat containing peroxides and aldehydes with peroxide number of the food 8.0 meq O2/kg. The clinical signs of experimental muscle dystrophy in broiler turkeys 2
appeared first by the 25th day of feeding, when the mild clinical form (II A group) and the severe clinical form (II B group) werewas established. The results indicated clearly that in turkey broilers suffering from muscle dystrophy, the concentrations of sulfur-containing amino acids cysteine and methionine decreased. After the treatment of turkey broilers with muscle dystrophy, and supplementation with non-deficient forage with Se, vitamin E, but also with sulfurcontaining amino acids cysteine or methionine with Seled at a dosage 0.06 mg/kg, the plasma levels of sulfur-containing amino acids cysteine and methionine was normalized in the mild clinical form (II A group). The birds affected by the severe clinical form of disease (II B group), which were not treated with Seled® and whose deficient feed was not corrected, could not recover and levels of sulfur-containing amino acids cysteine and methionine did not normalize.