Histology, serum biochemistry and haematological profiles of Clarias gariepinus fed diets containing Luffa cylindrica seedmeal

W.A. Jimoh1*, M.O. Shittu2, A.A. Ayeloja1, S.A. Abdulsalami3

1Department of Aquaculture and Fisheries, Faculty of Agriculture, University of Ilorin, PMB 1515, Ilorin,

Kwara State, Nigeria

2Department of Fisheries Technology, Federal College of Animal Health and Production Technology,

PMB 5029, Ibadan, Oyo State, Nigeria

3Fisheries and Aquaculture Unit, Department of Biological Sciences, Crescent University, Abeokuta,

Ogun State, Nigeria

(Manuscript received 24 December 2019; accepted for publication 26 March 2020)

Abstract. A 56-day feeding trial was conducted to evaluate the effect of processing time, inclusion level and/or their interactions on serum biochemistry, haematology and histology of the liver and kidney of Clarias gariepinus fed diets containing Luffa cylindrica seedmeal. The five formulated diets were designated as control (CTR) and tested, replaced at 15 and 30% by 5- and 10-min toasted Luffa cylindrica seedmeal (D515T, D530T, D1015T and D1030T). The experimental design followed a 2×2 factorial experiment in a completely randomised design; the processing time of 5- and 10-min toasting and inclusion level of 15 and 30% serves as factors. Triplicate groups of each treatment were made. Blood sampling, harvesting of organs, serum biochemistry, histology and haematological studies followed standard procedures. The results of the study showed that the effect of processing time, inclusion level and their interactions had significant impact (p<0.05) on Clarias gariepinus. A decrease in RBC, PCV and Hb was observed when compared with control for inclusion level, processing time and/or their interaction. Total protein, albumin and globulin of the blood of fish fed diet CTR was not significantly different (p>0.05) from that of the blood of the fish fed D1015T. Mild to moderate vacuolation of the hepatocytes were recorded among the livers of fish fed control and test dietary treatments except those fed D1030T that recorded very severe vacuolation of the hepatocytes. The kidney, being the excretory organ, was also affected. However, normal cell architecture was recorded in fish fed CTR, D530T and D1030T.