Superovulation and embryo transfer in goats by using PMSG and FSH

A. Pampukidou, T. Alifakiotis, M. Avdi, R. Ivanova

Abstract. The objective of this study was to analyze the efficiency of superovulation, embryo collection, transfer and embryo survival in indigenous goat during breeding season. Twenty-four goats-donors were synchronized by placing an intravaginal sponge of medroxyprogesterone acetate (MAP, 60mg) for 17 days. Superovulation was induced in 12 goats by a single injection of PMSG (1200 IU/animal, i.m.), at the time of sponge removal, and in 12 goats by FSH i.m. injections, twice a day, in a total amount of 21mg in reduced consecutive doses, starting at day 15 of the estrus cycle. All donors showing estrus, were bred naturally and 6 days post mating were subjected to a surgical uterine flushing with Dulbecco`s phosphate saline enriched with 3% BSA and fresh embryos were transferred surgically to synchronized recipients. Mean interval to onset of estrus did not differ between the two treatments; however the duration of estrus was shorter in FSH – treated group. There was significant difference (p<0.05) between treatments in total ovarian response, in ovulation rate (3.9 ± 0.9 group PMSG, and 12.3 ± 2.0, group FSH) and in embryo recovery (2.3 ± 0.8 for PMSG, and 9.3 ± 1.5 for FSH group). Embryo survival after transfer of embryos was significantly (p<0.05) higher for the FSH group (61.1% and 22.2% for FSH-p, and PMSG).