Abstract. The study comprises 143 Black-and-White cows from three cattle farms with free cubicle rearing (one) and tied rearing (two). The number of cows by farms is as follows: farm 1 – 69 cows, farm 2 – 34 cows and farm 3 – 40 cows. All cows in lactation from Ist to VIIth at the time of evaluation have been included, respectively in Ist lactation – 40 cows, in IInd – 28 and in IIIrd and further lactation – 75 cows. To assess the temperament a 5-point scale has been used, respectively 1 – very nervous and 5 – very calm cows. Recording the milking temperament has been done once during lactation and all cows currently in lactation on the three farms have been assessed. Primiparous up to the 30th day after calving have not been included in the assessment. Statistically significant effect of the farm on the milking temperament score has not been reported. In the three studied farms relatively high, above 80% share of calm cows has been reported – scores from 3 to 5. The highest is the relative share of nervous animals (scores 1 and 2) of farm 2 – 17.6%, and the lowest – of farm 3 – 12.5%. Statistically significant effect of the number of lactation on the milking temperament score has been reported. The highest is the percentage of nervous animals – 22.5% in cows at first lactation and, respectively, the lowest is the share of the calm ones – 77.5%. With cows after Ist lactation the percentage of nervous cows is half less and it remains almost unchanged in later lactations. The highest is the LS-mean score for the temperament of cows in IIIrd and further lactations – 4.15, followed by those in IInd. The lowest is the LS-mean score for temperament of cows in first lactation – 3.82.